Fluorescence-polarization spectrum and electronic-energy transfer in proteins.
نویسنده
چکیده
منابع مشابه
Polarized absorption spectra of green fluorescent protein single crystals: transition dipole moment directions.
Polarized absorption spectra of orthorhombic crystals of wild-type green fluorescent protein (GFP) were measured between 350 and 520 nm to obtain information on the directions of the electronic transition dipole moments ((-->)m) of the chromophore relative to the molecular axes. The transition dipole moment orientation is a basic spectroscopic parameter of relevance to biologists when interpret...
متن کاملExcitation Energy Transfer among Chlorophyll a Molecules in Polystyrene: Concentration Dependence of Quantum Yield, Polarization and Lifetime of Fluorescence
Electronic excitation energy transfer was studied for chlorophyll a in a solid solution of poly styrene by measuring the concentration quenching of quantum yield, polarization, and lifetime of fluorescence. The concentration quenching of the experimental fluorescence quantum yield is adequately described by Kelly and Porter’s empirical formula (Proc. Roy. Soc., Lond. A 315, 149, 1970), and of ...
متن کاملElectronic Excitation Transfer from Carotenoid to Bacteriochlorophyll in the Purple Bacterium <italic>Rhodopseudomonas acidophila</italic>
Ultrafast fluorescence upconversion has been used to probe electronic excitation transfer within the B800B820 light-harvesting antenna of Rhodopseudomonas acidophila strain 7050. Emission from the carotenoid S2 band decays in 54 ( 8 fs, and the bacteriochlorophyll B820 Qy band rises in approximately 110 fs. The B820 Qy rise time is wavelength-dependent. Energy-transfer rates between the caroten...
متن کاملImaging molecular interactions in cells by dynamic and static fluorescence anisotropy (rFLIM and emFRET).
We report the implementation and exploitation of fluorescence polarization measurements, in the form of anisotropy fluorescence lifetime imaging microscopy (rFLIM) and energy migration Förster resonance energy transfer (emFRET) modalities, for wide-field, confocal laser-scanning microscopy and flow cytometry of cells. These methods permit the assessment of rotational motion, association and pro...
متن کاملAntagonistic Effects of Mono-and Divalent Cations on Polarization of Chlorophyll Fluorescence in Thylakoids and Changes in Excitation Energy Transfer
Monoand divalent cations affect several primary photoprocesses in thylakoids: initial energy distribution between the two pigment systems [1 -7 ] , excitation energy redistribution or 'spillover' from pigment system (PS) II to PSI [2,4,6-15], rate constant of thermal dissipative transitions [6,7,16,17], and activation of reaction center II [18-20] . Several of these effects may occur concurrent...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- The Biochemical journal
دوره 75 شماره
صفحات -
تاریخ انتشار 1960